Project
Part:BBa_K596000:Design
Designed by: Ufuk Değirmenci Group: iGEM11_Bilkent_UNAM_Turkey (2011-06-30)
Nitroreductase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 387
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 387
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 152
Illegal XhoI site found at 254 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 387
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 387
Illegal NgoMIV site found at 16
Illegal AgeI site found at 854 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It contains PstI sequence in open reading frame. This site must be deleted.
Source
This sequence derived from Enterobacter cloacae.